Lamide gel electrophoresis, and transferred onto a nitrocellulose membrane (Amersham Biosciences
Lamide gel electrophoresis, and transferred onto a nitrocellulose membrane (Amersham Biosciences, Piscataway, NJ). Next, immunoblot analysis was performed working with anti-MMP-9 antibody (Millipore, Billerica, MA), as well as anti-ZO-1 and…