Le’s medium (DMEM, Life Technologies, Alcobendas, Spain), pH 7.four, supplemented with
Le’s medium (DMEM, Life Technologies, Alcobendas, Spain), pH 7.four, supplemented with 10 fetal calf serum (Life Technologies), ten mM HEPES, 40 mM NaHCO3, one hundred units/ml penicillin, and one hundred…