,14Z,17Z-20:three) Dihomo- -linolenic (8Z,11Z,14Z-20:three) Eicosadienoic (11Z,14Z-20:two) 5-Eicosenoic (5Z-20:1) 8-Eicosenoic (8Z-20:1) 11-Eicosenoic (11Z-20:1) Arachidic (20:0) Docosahexaenoic (4Z,7Z,10Z,13Z,16Z,19Z-22:six) Docosapentaenoic (7Z,10Z,13Z,16Z,19Z-22:five) Docosapentaenoic (4Z,7Z,10Z,13Z,16Z-22:5) Docosatetraenoic (7Z,10Z,13Z,16Z-22:four) Docosatrienoic (13Z,16Z,19Z-22:three) Docosadienoic (13Z,16Z-22:2) Erucic (13Z-22:1) Behenic (22:0) Nervonic (15Z-24:1) Lignoceric (24:0) Internal Requirements (A) D14 palmitoleic (9Z-16:1) (B) D31 palmitic (16:0) (C) D14 -linolenic (9Z,12Z,15Z-18:3) (D) D4 linoleic (9Z,12Z-18:two) (E) D17 oleic (9Z-18:1) (F) D5 eicosapentaenoic (5Z,8Z,11Z,14Z,17Z-20:5) (G) D35 stearic (18:0) (H) D8 arachidonic (5Z,8Z,11Z,14Z-20:four) (I) D6 Dihomo- -linolenic (8Z,11Z,14Z-20:three) (J) D5 Docosahexaenoic (4Z,7Z,10Z,13Z,16Z,19Z-22:six)4.37 4.96 five.35 six.04 6.34 six.5 7.02 five.eight 6.21 6.29 six.7 six.96 7.26 7.26 7.26 7.91 6.26 six.73 6.6 7.07 7.07 7.53 eight.29 eight.15 8.08 8.74 6.74 6.98 6.98 7.41 7.88 eight.33 eight.85 9.51 9.59 10.22 6.3 six.92 6.17 six.75 7.28 6.31 7.81 six.77 7.04 six.1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 2 1 1 1 1 1 1 two two 2 2 1 1 1 1 2 two two 2 2 2 1 1 1 1 1 1 two 1 1A A A A A A B A C C D D E E E G F H H I I E G G G G J J J J G G G G G G A B C D E F G H I J365 367 393 395 421 421 423 443 445 445 447 447 449 449 449 451 469 471 471 473 473 475 477 477 477 479 495 497 497 499 501 503 505 507 533 535 435 455 459 451 466 474 487 479 479239 239 239 239 239 239 239 239 239 337 239 347 239 239 239 239 239 239 239 239 373 239 363 239 365 239 375 239 239 239 239 239 239 239 239 239 239 239 239 239 239 239 242 242 242 239 239 239 242 239 23956 60 58 62 60 60 65 64 64 64 64 64 65 65 64 64 64 66 62 70 70 70 70 70 70 70 70 65 70 70 70 66 64 65 65 65 65 65 65 65 70 65 60 65 64 68 68 62 65 65 7042 44 44 47 47 47 49 42 46 38 45 38 48 48 45 45 45 45 42 50 40 50 40 50 40 50 38 52 50 50 50 55 36 55 55 55 55 55 55 55 60 55 47 55 48 44 48 43 58 45 46Fatty acyl chains are abbreviated with the number of carbons and double bonds as well as the double bond positions, i.187039-57-2 Formula e.Lumisterol 3 (>90%) Purity five,9-18:two is definitely an 18 carbon fatty acyl chain with 2 double bonds beginning at carbons five and 9 from the carboxyl finish. All double bonds have the cis (Z) configuration unless stated otherwise. a Retention occasions listed are derived in the LC protocol detailed within the Approaches section. b Data for retention window 1 was collected from minutes 4.0 to 7.65. Data for retention window two was collected from minutes 7.65 to ten. c The m/z values listed are calculated monoisotopic values. The actual center mass values applied are derived from instrument tuning, which is instrument dependent. d Cone voltages and collision energies were optimized for each and every analyte. These numbers are instrument dependent.Journal of Lipid Research Volume 54,Optima grade, catalog #A117-50), and solvent B was acetonitrile (Fisher Optima grade, catalog #L-14338)/0.PMID:33722183 1 formic acid. The solvent system was (linear gradients): 0?.5 min, 90 A; 0.five?.51 min, 90?0 A; 0.51?0.0 min, 80?0 A; ten.0?0.1 min, 30? A; 10.1?two.0 min, 0 A; 12.0?two.1 min, 0?0 A; 12.1?5.0 min, 90 A. The flow rate was 0.4 ml/min and column temperature was 45 . Supplementary Table I summarizes the auto-sampler and ESI-MS/MS parameters for data collection, respectively.Outcomes AND DISCUSSIONAs noted within the introduction, conversion with the carboxyl group of lipids, such as eicosanoids and FAs, for the AMPP amide results in an analyte with a permanent positive charge, which may be analyzed by LC-ESI-MS/MS in positive.